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Infections following the application of leeches: two case reports and writing anne frank. review of the literature. © Maetz et al.; licensee BioMed Central Ltd. 2012. Published: 25 October 2012. Since the 1980s, leeches have been ingeniously used in the management of venous flap congestion. The presence of anticoagulative substances in their saliva improves the blood drainage. Writing Australian Nc State. Their digestive tract contains several bacterial species, the main ones being Aeromonas hydrophila and Aeromonas veronii biovar sobria, which contribute to the digestion of ingested blood. These bacteria can be the paper frank., cause of infections. We report two cases of custom septicemia related to Aeromonas veronii biovar sobria that presented after leeches had been applied to congested transverse rectus abdominis myocutaneous flaps for my research paper frank. delayed mammary reconstructions. Patient number 1 was a 55-year-old Caucasian woman who underwent a delayed breast reconstruction procedure. On the sixth postoperative day she showed a clinical presentation of septicemia.

Aeromonas veronii biovar sobria was identified in the patient’s skin and blood bacteriological samples. Her fever ceased after 4 days of antibiotic treatment. Patient number 2 was a 56-year-old Caucasian woman who underwent a delayed breast reconstruction procedure. Three Of The Categorical. On the seventh postoperative day we noticed that she showed a clinical presentation of septicemia. Aeromonas veronii biovar sobria was identified in the patient’s blood cultures and local bacteriological samples. An antibiogram showed resistance to amoxicillin/clavulanic acid. Her fever ceased on the eleventh postoperative day after 4 days of antibiotic treatment. The rate of infection after application of leeches is not negligible.

The concentration of frank. Aeromonas inside the custom australian dialect coach nc state, digestive tracts of writing paper leeches largely decreases when the The Rogerian Research Paper, patient is under antibiotic therapy. These germs are sensitive to my research paper anne, third-generation cephalosporins and fluoroquinolones and resistant to amoxicillin/clavulanic acid. We recommend preventive treatment based on classical measures of asepsis and on oral antibioprophylaxy with a fluoroquinolone during the how to, whole period of treatment by leeches. Leeches have been used for my research paper anne therapeutic purposes since 3500 B.C. Their qualities were discovered in former Egypt, China and India. To Scholarship Essay Questions. A mural painting representing a nurse applying a leech to a patient’s forehead was discovered in a grave in Thebes (origin of the pharaohs of the 18th dynasty). The first written reference goes back to the second century B.C. and deals with the paper anne, treatment of poisonous bites. The use of leeches reached a peak in the nineteenth century for various indications: laryngitis, ophthalmic problems, cerebral apoplexy, obesity, and mental disorders. In 1884, Haycraft discovered hirudin which is the help me do my essay categorical, main anticoagulative substance in my research paper anne frank., leeches’ saliva [ 1 ]. In 1955, Markwardt was the first to isolate hirudin from the pharyngeal glands of buy essay of the united states leeches. Nowadays, this substance is only produced by genetic engineering.

It was in 1981 that Foucher described for the first time the use of leeches in the treatment of venous congestion during digital replantation [ 2 ]. Since then leeching has been used particularly for paper frank. the treatment of venous drainage problems [ 3 ]. Leeches are divided hermaphrodite worms that feed on blood and they belong to the group of annelids. Many species are known and the two most commonly used in medical application are the Hirudo medicinalis and Hirudo verbana [ 4 ] . Their length is paper, about 12cm and writing paper anne frank. their weight is 2g to 5g. The mouth of the leech has three jaws; each jaw has approximately 100 tiny teeth. A leech also has a posterior suction device that it uses for stability. How To To Scholarship. Medicinal leeches have two different mechanisms through which venous drainage can be achieved. My Research Anne Frank.. The first mechanism is through the passive bleeding of the patient after each leech bite; this represents the majority of the average blood meal volume for a leech. They temporarily increase perfusion levels by how to essay, actively drawing off blood and maintain physiologic requirements within the congested tissue. Writing My Research Paper Anne Frank.. Laser Doppler flowmetry can demonstrate a significant increase in superficial skin perfusion around the leech bite [ 5 , 6 ]. Moreover, as the leech bite continues, it reduces congestion due to the anticoagulant effect of leech saliva, which contains thrombin inhibitor hirudin, apyrase, collagenase, hyaluronidase, factor Xa inhibitor and fibrinase I and II [ 2 ]. A leech consumes 5mL to custom essay australian dialect coach, 15mL of blood and induces oozing on the site of attachment of between 50mL and 100mL of blood during the 24-hour to anne, 48-hour period after the leech is detached. Venous congestion treated by leeches. In France, medicinal leeches are supplied by Ricarimpex®.

Before being used, leeches are kept in a bowl of sterile water under refrigeration. It is recommended to writing template, store leeches at writing my research anne frank., a cool temperature ranging from 5°C to 7°C (42°F to 45°F) and no higher than 20°C (68°F). The water should be changed every week and college entrance essay a bacteriological sample taken once a month. Leeches’ intestinal flora is composed of Aeromonas hydrophila and Aeromonas veronii biovar sobria [ 8 ] making the digestion of sampled red blood corpuscles easier. My Research. Since 1983, many infections due to these germs have been described after medical application [ 9 ]. We report two cases of septicemia related to A. veronii that presented after leeches had been applied to congested transverse rectus abdominis myocutaneous (TRAM) flaps for delayed mammary reconstructions before reviewing the international literature on the topic. The first patient was a 55-year-old Caucasian woman who underwent a mastectomy for a breast cancer followed by radiotherapy and chemotherapy. Three years after those treatments, she underwent a delayed breast reconstruction procedure with a pedicled TRAM flap. Due to a massive and immediate venous congestion, she had to my essay three categorical imperative, undergo 12 hours later a second operation and the flap had to be moved back to the initial position.

Due to persistent venous congestion, a treatment with leeches was started 2 days after this second operation. Neither an intraoperative nor a patient-related risk factor could explain the observed venous congestion. On the sixth postoperative day, following 4 days of leeching, the vascular status of the flap improved, but the my research paper frank., patient showed a clinical presentation of septicemia associated with 39.2°C hyperthermia, a white blood cell count of me do formulations of the categorical imperative 27,44 10 9 /L and a C-reactive protein (CRP) level increased by writing paper anne frank., 153mg/L. While blood cultures and local bacteriological swabs were being analyzed, an intravenous antibiotic therapy was started including vancomycin (1.5g/day) and cefotaxime (6g/day) for 10 days, and amikacin (350mg/day) for 2 days. A. veronii biovar sobria was identified in skin and The Rogerian Research blood bacteriological samples. The fever ceased after 4 days of writing my research anne frank. antibiotic treatment. After 10 days, oral clindamycin antibiotic therapy was prescribed for 10 days. The flap was moved back to the mammary position at day 12 after 48 hours of apyrexia. The patient left the hospital at day 19. The second patient was a 56-year-old Caucasian woman who underwent a mastectomy for spider writing template a relapse after a first breast cancer treated by tumorectomy followed by my research frank., radiotherapy. Spider Writing Template. Despite the my research paper frank., possibilities of immediate breast reconstruction, she refused to buy essay online cheap the history states constitution, undergo such a procedure.

Ten months later, she was motivated for a delayed procedure. Because we were not used to performing deep inferior epigastric perforator flaps at writing, that time, she had a breast reconstruction using a pedicled TRAM flap. Despite the spider writing paper template, absence of intraoperative complications and patient-related risk factors, the flap presented an early venous congestion that was treated from the second to the fifth postoperative day by anne, leeching and heparin-moist gauzes. A partial necrosis settled in the mammary and abdominal area. On the seventh postoperative day, we noticed a clinical presentation of septicemia associated with 39.8°C hyperthermia, a white blood cell count of 15 ? 10 9 /L and a CRP level increased by 114mg/L. On the eighth postoperative day, a necrotic area excision was practiced, associated with an intravenous probabilistic antibiotic therapy including amoxicillin/clavulanic acid (4g/day) and gentamicin (160mg/day) for 2 days. A. veronii biovar sobria was identified in the patient’s blood cultures and local bacteriological samples. An antibiogram showed resistance to amoxicillin/clavulanic acid, which was stopped at good, day 10 and replaced by cefotaxime (6g/day) for 10 days followed by paper frank., oral ofloxacin (400mg/day) for eight days. The fever ceased on the eleventh postoperative day after four days of antibiotic treatment. She left the hospital at college, day 20.

For our two patients, the sample was plated to blood agar, Drigalski agar and a brain-heart infusion. The isolate was identified by using a Vitek 2 Gram-negative card (bioMerieux Inc., Marcy L’etoile, France). Antimicrobial susceptibility was tested by using a 24-hour disk-diffusion method on writing my research paper anne frank., Mueller-Hinton agar plates incubated at how to to scholarship essay questions, 37°C in an ambient air incubator. Similar cases of infections due to leeching on TRAM flaps have been described in the literature [ 10 ]. It was in 1983 that Whitlock et al . suggested for my research paper anne the first time that there could be a risk of infection by A. Essay Writing. hydrophila attached to leeching. Whitlock showed the presence of A. hydrophila on the leech’s body by making bacteriological samples.

These bacteria can be pathogenic for the human body especially when the flap has a bad vascularization [ 9 ]. In fact, each leech’s bite potentially introduces the bacteria into tissue with poor microcirculation, setting the stage for a possible infection. In the writing my research, literature, many cases of infection after leeching have been described [ 11 ]: 18 cases in of the united states constitution, 1992 by Lineaweaver [ 12 ], 19 cases in 1996 by De Chalain [ 13 ], 7 cases in 2002 by Sartor [ 14 ], and 47 cases in 2007 by Bauters [ 15 ]. The rate of my research frank. infection can be important varying from 2.4% to 20.0% according to the literature: 17% in De Chalain [ 13 ] and 3% in Sartor’s cases [ 14 ]. De Chalain et al. [ 13 ] described 19 cases of the history states constitution leeche-related Aeromonas infection. Writing. The flap salvage rate in infected patients dropped to 30% compared to an 83% flap survival rate in custom writing australian coach nc state, uninfected patients. The severity ranges from mild cellulitis and trivial episodes of wound drainage to more serious infections with abscess, tissue necrosis, septicemia and meningitis [ 16 ]. Several infections were observed at the donor site and at other sites far from the flap [ 12 ]. Unlike our two patients, in general, many patient-related risk factors were found that could explain the my research anne frank., infection rate: immune deficiency, diabetes, hepatobiliary disease (cirrhosis), obesity, and good college essay tobacco use. Infection starts most of the writing my research paper anne, time during the first 10 days, but in help me do three formulations of the imperative, some cases infection can appear a few weeks after leeching [ 16 ]. In our two cases, the delay between leeching and the beginning of septicemia ranges from 4 to 7 days. In Lineaweaver’s [ 12 ] series of 18 patients infected after leeching, this delay ranges from 1 day to 10 days.

In Sartor’s series [ 14 ] of seven patients infected after leeching, this delay ranges from 2 days to 11 days. Numerous germs have been identified from writing paper, leeches [ 17 ]. Writing. The saliva from H. Frank.. medicinalis, which is the most commonly used medicinal leech in Europe and Northern America, was put into culture by Mackay et al . and they showed the me do three formulations imperative, presence of A. hydrophila and Aeromonas sobria [ 18 ]. The most common germ is A. hydrophila but other pathogens that cause wound infections following leeching include A. sobria [ 19 ], and there are isolated reports of Serratia marcescens [ 20 ] and Vibrio fluvialis [ 21 ]. A. veronii biovar sobria was found in blood cultures as well as in local bacteriological samples of our two patients. R: Resistant, S: Sensitive. Before use and in order to avoid human infection, some authors propose immersing leeches in a solution of antibiotics for a few days or in a solution of chlorhexidine at 1:5000 before therapeutic use. Use of povidone iodine is excluded due to its absolute toxicity on leeches.

Hokelek et al . consider that incubation of leeches with the appropriate antibiotics before application may contribute to prevent patients’ infection [ 24 ]. Leeches’ suction activity is preserved notwithstanding this treatment. Writing My Research Paper Frank.. Different solutions of antibiotics were compared and analyzed; their efficiency and their impact on bacterial concentration inside the leeches’ digestive tract was studied. The results showed that optimum eradication of bacteria from leeches was obtained with a dosage of 500?g per mL of college entrance essay ciprofloxacin and 1000?g per mL of frank. ceftriaxone. Hokelek proposed the use of a solution of antibiotics with ciprofloxacin that was superior to ceftriaxone. Despite these preventive measures, some infectious cases were identified. In fact, no solution of antibiotics can totally eradicate the writing, bacterial flora from the digestive tracts of leeches. Lineaweaver [ 25 ] nevertheless noticed an important decrease in the concentration of bacteria inside the digestive tracts of leeches when the patient is under an writing anne frank., effective antibiotic therapy. Consequently, only the patients’ antibioprophylaxy is able to Research Paper Research, reduce the risk of infection.

In 2002, Chepeha et al . [ 26 ] used double coverage with ciprofloxacin and trimethoprim/sulfamethoxazole prophylaxis in eight patients treated for venous congestion with an writing my research frank., average of 215 leeches per custom writing australian patient without any Aeromonas infection reported. In 2004, Whitaker et al . [ 27 ] proposed a prophylactic protocol for frank. the patient based on an intravenous antibioprophylaxy associating a fluoroquinolone such as ciprofloxacin and an aminosid during the online the history states constitution, complete period of leeching. Writing Frank.. This treatment is template, followed by an oral one until complete healing is achieved. Writing Anne. In 2007, Knobloch et al . Writing Paper. [ 28 ] recommended a prophylactic antibiotic therapy with fluoroquinolone antibiotics such as 500mg of ciprofloxacin three times a day for writing paper anne frank. 7 days. In the presence of custom writing coach necrotic tissue or an writing my research paper anne, open wound, oral antibiotic cover should be continued until wound closure. Effective antibiotic treatment may reduce the college, possibility of Aeromonas colonization of a devitalized portion of tissue and writing paper prevent late A. Custom Writing Dialect Coach Nc State. hydrophila infection [ 29 ]. Established infection is treated with antibiotics such as third-generation cephalosporins, along with aminoglycosides, fluoroquinolones, tetracycline, or trimethoprim/sulfamethoxazole [ 29 ]. As for our two patients, Hermansdorfer’s and paper frank. Braga’s studies reveal that the Aeromonas is sensitive to fluoroquinolone, particularly ofloxacin and ciprofloxacin [ 23 , 30 ]. Ciprofloxacin is the most recommended prophylactic antibiotic for leech therapy because studies have consistently shown 100% sensitivity of Aeromonas strains isolated from medicinal leeches.

So, an antibioprophylaxy with ofloxacin at 400mg/day or with ciprofloxacin 1g/day efficiently reduces the infection rate during the whole period of treatment. Tissue penetration is good and side effects are limited. Ofloxacin has an excellent oral bioavailability (close to 95%) and a lower cost considering the relatively few cases in which leeches are employed [ 31 ]. In 2011, Wang et al . described a ciprofloxacin and trimethoprim/sulfamethoxazole-resistant Aeromonas infection associated with leech therapy [ 32 ]. Studies that examined the antibiotic resistance profile of Aeromonas strains in custom writing australian nc state, industrial fish farms found a resistance to paper, ciprofloxacin and to spider template, trimethoprim/sulfamethoxazole estimated to be 3% and my research paper 1%, respectively [ 33 , 34 ]. Currently, ciprofloxacin and The Rogerian Paper trimethoprim/sulfamethoxazole resistances in Aeromonas are rare. In order to determine appropriate antibiotic prophylaxis on local resistance patterns, surgeons and writing my research paper anne frank. infectious disease specialists have to collaborate. The increase in fluoroquinolone resistance needs to be cautiously monitored. The rate of Research Paper Research Paper infection after leeching is not negligible. It varies from writing my research anne frank., 2.4% to 20.0% according to the literature [ 11 , 12 ]. The concentration of buy essay online the history of the united constitution Aeromonas inside the digestive tracts of leeches largely decreases when the patient is under antibiotic therapy. These germs are sensitive to third-generation cephalosporins and fluoroquinolones such as ofloxacin. Frank.. We recommend preventive treatment based on classical measures of how to asepsis and on oral antibioprophylaxy with fluoroquinolones such as ofloxacin at 200mg twice a day during the whole period of treatment by my research, leeches. Written informed consent was obtained from the two patients for publication of good college these case reports and any accompanying images.

A copy of the written consent is available for writing paper anne frank. review by to scholarship, the Editor-in-Chief of this journal. Authors' original submitted files for images. The authors declare that they have no competing interests. BM and RA were involved in patients’ management and wrote the manuscript. Frank.. CBR also reviewed the literature. CBR operated on three formulations categorical imperative, the first patient and JBA on the second one. All authors read and writing frank. approved the final manuscript. Whitaker IS, Rao J, Izadi D, Butler PE: Historical Article: Hirudo medicinalis: ancient origins of, and trends in the use of medicinal leeches throughout history. Br J Oral Maxillofac Surg. 2004, 42 (2): 133-137. 10.1016/S0266-4356(03)00242-0.

View ArticlePubMedGoogle Scholar Foucher G, Henderson H, Maneaud M, Merle M, Braun FM: Distal digital replantation: one of the best indications for microsurgery. Int J Microsurg. 1981, 36: 240-243. Google Scholar Batchelor AG, Davison P, Sully L: The salvage of congested skin flaps by the application of leeches. Br J Plast Surg.

1984, 37: 358-360. 10.1016/0007-1226(84)90079-1. View ArticlePubMedGoogle Scholar Siddall ME, Trontelj P, Utevsky SY, Nkamany M, Macdonald KS: Diverse molecular data demonstrate that commercially available medicinal leeches are not Hirudo medicinalis. Proc Biol Sci. 2007, 274 (1617): 1481-1487.

10.1098/rspb.2007.0248. View ArticlePubMedPubMed CentralGoogle Scholar Hayden RE, Phillips JG, McLear PW: Leeches. Objective monitoring of altered perfusion in how to response to scholarship questions, congested flaps. Arch Otolaryngol Head Neck Surg. 1988, 114 (12): 1395-1399. Writing My Research Anne Frank.. 10.1001/archotol.1988.01860240045021.

View ArticlePubMedGoogle Scholar Scnabl SM, Kuntz C, Unglaub F, Polykandriotis E, Horch RE, Dragu A: Acute postoperative infection with Aeromonas hydrophila after using medical leeches for treatment of good entrance essay venous congestion. Arch Orthop Trauma surg. 2010, 130 (10): 1323-1328. 10.1007/s00402-010-1135-y. View ArticleGoogle Scholar Yantis MA, O’Toole KN, Ring P: Leech therapy. Am J Nurs. 2009, 109 (4): 36-42. Writing Anne. 10.1097/01.NAJ.0000348601.01489.77. Paper Template. quiz 43 View ArticlePubMedGoogle Scholar Graf J: Symbiosis of writing Aeromonas veronii biovar sobria and Hirudo medicinalis. The medicinal leech: a novel model for digestive tract associations.

Infect Immun. 1999, 67 (1): 1-7. PubMedPubMed CentralGoogle Scholar Whitlock MR, O'Hare PM, Sanders R, Morrow NC: The medicinal leech and help three of the its use in plastic surgery: a possible cause for infection. Br J Plast Surg. 1983, 36 (2): 240-244. My Research Frank.. 10.1016/0007-1226(83)90100-5. View ArticlePubMedGoogle Scholar Bourdais L, Heusse JL, Aillet S, Schoentgen C, Watier E: Leech-borne infection on a TRAM flap: a case report.

Ann Chir Plast Esthet. 2010, 55 (1): 71-73. 10.1016/j.anplas.2009.05.001. View ArticlePubMedGoogle Scholar Whitaker IS, Oboumarzouk O, Rozen WM, Naderi N, Balasubramanian SP, Azzopardi EA, Kon M: The efficacy of medicinal leeches in plastic and reconstructive surgery: a systematic review of 277 reported clinical cases. Microsurgery. 2012, 32 (3): 240-250. Essay. 10.1002/micr.20971. View ArticlePubMedGoogle Scholar Lineaweaver WC, Hill MK, Buncke GM, Follansbee S, Buncke HJ, Wong RK: Aeromonas hydrophila infections following use of writing paper frank. medicinal leeches in replantation and flap surgery. Writing Australian. Ann Plast Surg. 1992, 29 (3): 238-244. 10.1097/00000637-199209000-00008.

View ArticlePubMedGoogle Scholar De Chalain TM: Exploring the my research anne, use of the medicinal leech: a clinical risk-benefit analysis. J Reconstr Microsurg. 1996, 12 (3): 165-172. 10.1055/s-2007-1006471. View ArticlePubMedGoogle Scholar Sartor C, Limouzin-Perotti F, Legre R, Casanova D, Bongrand MC, Sambuc R: Nosocomial infections with Aeromonas hydrophila from leeches. Clin Infect Dis. 2002, 35 (1): E1-E5. 10.1086/340711. View ArticlePubMedGoogle Scholar Bauters TG, Buyle FM, Verschraegen G, Vermis K, Vogelaers D, Claeys G: Infection risk related to The Rogerian Paper, the use of medicinal leeches. Writing My Research Paper Anne Frank.. Pharm World Sci.

2007, 29 (3): 122-125. 10.1007/s11096-007-9105-3. View ArticlePubMedGoogle Scholar Ouderkirk JP, Bekhor D, Turett GS, Murali R: Aeromonas meningitis complicating medicinal leech therapy. Clin Infect Dis. Essay. 2004, 38 (4): e36-e37. Writing My Research Paper Frank.. 10.1086/381438. View ArticlePubMedGoogle Scholar Whitaker IS, Kamya C, Azzopardi EA, Graf J, Kon M, Lineaweaver WC: Preventing infective complications following leech therapy: is practice keeping pace with current research?.

Microsurgery. 2009, 29 (8): 619-625. 10.1002/micr.20666. View ArticlePubMedGoogle Scholar Mackay DR, Manders EK, Saggers GC, Banducci DR, Prinsloo J, Klugman K: Aeromonas species isolated from medicinal leeches. United States Constitution. Ann Plast Surg. Writing My Research Paper Anne Frank.. 1999, 42 (3): 275-279. 10.1097/00000637-199903000-00008.

View ArticlePubMedGoogle Scholar Fenollar F, Fournier PE, Legre R: Unusual case of Aeromonas sobria cellulitis associated with the use of leeches. Eur J Clin Microbiol Infect Dis. 1999, 18 (1): 72-73. 10.1007/s100960050232. View ArticlePubMedGoogle Scholar Pereira JA, Greig JR, Liddy H, Ion L, Moss AL: Leech-borne Serratia marcescens infection following complex hand injury. Br J Plast Surg. 1998, 51 (8): 640-641.

10.1054/bjps.1998.0128. View ArticlePubMedGoogle Scholar Varghese MR, Farr RW, Wax MK, Chafin BJ, Owens RM: Vibrio fluvialis wound infection associated with medicinal leech therapy. Clin Infect Dis. 1996, 22 (4): 709-710. View ArticlePubMedGoogle Scholar Haycox CL, Odland PB, Coltrera MD, Raugi GJ: Indications and complications of medicinal leech therapy. J Am Acad Dermatol. 1995, 33 (6): 1053-1055. 10.1016/0190-9622(95)90320-8. View ArticlePubMedGoogle Scholar Hermansdorfer J, Lineaweaver W, Follansbee S, Valauri FA, Buncke HJ: Antibiotic sensitivities of The Rogerian Research Aeromonas hydrophila cultured from medicinal leeches. Br J Plast Surg. 1988, 41 (6): 649-651.

10.1016/0007-1226(88)90176-2. My Research Frank.. View ArticlePubMedGoogle Scholar Hokelek M, Guneren E, Eroglu C: An experimental study to custom writing dialect coach nc state, sterilize medicinal leeches. Eur J Plast Surg. 2002, 25: 81-85. 10.1007/s00238-002-0348-x. Writing Frank.. View ArticleGoogle Scholar Lineaweaver WC, Furnas H, Follansbee S, Buncke GH, Whitney TM, Canales F: Postprandial Aeromonas hydrophila cultures and antibiotic levels of enteric aspirates from medicinal leeches applied to patients receiving antibiotics. Ann Plast Surg. 1992, 29 (3): 245-249. The Rogerian Paper Research Paper. 10.1097/00000637-199209000-00009. View ArticlePubMedGoogle Scholar Chepeha DB, Nussenbaum B, Bradford CR, Teknos TN: Leech therapy for patients with surgically unsalvageable venous obstruction after revascularized free tissue transfer. Writing Paper Frank.. Arch Otolaryngol Head Neck Surg.

2002, 128 (8): 960-965. How To Questions. View ArticlePubMedGoogle Scholar Whitaker IS, Izadi D, Oliver DW, Monteath G, Butler PE: Hirudo medicinalis and the plastic surgeon. Br J Plast Surg. Paper. 2004, 57 (4): 348-353. 10.1016/j.bjps.2003.12.016. View ArticlePubMedGoogle Scholar Knobloch K, Gohritz A, Busch K, Spies M, Vogt PM: Hirudo medicinalis – leech applications in writing paper template, plastic and reconstructive microsurgery – a literature review. Handchir Mikrochir Plast Chir. 2007, 39 (2): 103-107.

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Int J Antimicrob Agents. 2007, 30 (2): 177-182. 10.1016/j.ijantimicag.2007.03.012. Custom Essay Writing Australian Dialect Nc State. View ArticlePubMedGoogle Scholar Penders J, Stobberingh EE: Antibiotic resistance of motile aeromonads in indoor catfish and eel farms in paper, the southern part of the Netherlands. Int J Antimicrob Agents. 2008, 31 (3): 261-265. 10.1016/j.ijantimicag.2007.10.002. View ArticlePubMedGoogle Scholar. This article is published under license to BioMed Central Ltd.

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