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bard of bressay Shetland: Descriptive and Historical. Part II: Chapter 12. Buy Research Paper Online Motoring Mischief? BRESSAY AND NOSS. East Coast of Bressay—Cave—Bard—“Giant’s Leg”—Holm and Neup of Noss—The Cradle—Dr Copland—Farm of Noss— Dangers of Noss Sound—Bressay again—Gar die—Maryfield —Parochial Statistics—Slate Quarries. AND now for a visit to some of the “lions” in the vicinity of Lerwick. The Cave of Bressay and the Cliffs of , Noss amply repay a day's excursion.

Steering southwards through the buy research paper online in indianapolis, Sound, and shaping our course along the Bressay shore, we pass, in succession, the mansion of Gardie, the services, house of Maryfield, the kirk, the manse, and the lighthouse, with various cottages and crofts interspersed between these buildings of greater note. Doubling the point of Kirkabister, and, if we are bold enough, sailing through a fine natural archway nearly underneath the lighthouse, we pass the buy research online, lofty Ord, and direct our bark eastward to the root of the Bard. Best Services? Here we find the cave, its entrance guarded by a skerry,1 not much above high-water mark. The entrance is a wonderfully symmetrical archway, pretty lofty, and paper online mischief, wide enough to admit several small boats abreast. Here the water is very clear and deep; and the display of colours on cant essay what you should about, the roof and walls remarkable both for brilliancy and variety. Our advance into buy research online motoring mischief the cavern is now attended with greater difficulty, as the passage becomes narrow and takes a curved direction. Soon the narrows are passed, and we find ourselves in a spacious hall. From its lofty ceiling hang numerous stalactites, many of them assuming very fantastic shapes. Pilasters of the same calcareous material have been formed on the walls.

So great is their hardness that it is difficult, even with the aid of the hammer and writing essay english, chisel, to detach pieces. Paper Online Mischief? In this sea-paved hall reigns Egyptian darkness, save when it is dispelled by the torch of the visitor. Here the smallest noise, even the papers online reputation, stroke of an oar, resounds like thunder. Beyond the hall we can penetrate to a considerable distance, but the inner reaches of the cavern become narrower and narrower. At length our skiff s arrested by a sea-beach, from paper mischief which a faint streak of light is to be discerned shining through a slit in the rock, a long way off. Beyond this, no mortal man is essay services pigmean enough to advance. On a shelf near the mouth of this weird helyr, a native of the sister archipelago is said to have hid from the paper motoring mischief, pressgang; and a historical english, hence, it is sometimes called the Orkneyman’s Cave. Emerging from it, we for a few minutes steer south-eastwards and reach the paper online motoring, Bard, in which Bressay terminates on the south. Here the most fantastic forms diversify the surface of the rocks. Nature has been the essay english, sculptor, employing the elements to remove the soft particles of which the cliffs are composed, while the online mischief in indianapolis, harder remain.

At the extreme point of the Bard, a narrow but somewhat lofty arch displays itself. The outer pier of this arch acts like a buttress to the precipice, which forms the inner. This curious buttress-like pillar is not inappropriately termed the Giant’s Leg. Tradition says that, ages since, a certain giant, in leaping from Shetland to Orkney, succeeded in essay services 50s diner getting one leg over, but left the other behind, and here it still stands at the Bard of Bressay. Advancing north-eastwards for three or four miles, we come to the far-famed Holm of Noss. This is a small islet one hundred and sixty feet high, flat and well clothed with grass on online mischief, the top, but precipitous on all sides.

On one side, its cliffs are lashed by the full fury of the services, North Sea; and on the other, its base is washed by the conflicting currents which agitate the narrow strait separating the Holm from the paper mischief, island of Noss. A little to the north of the Holm is the Noup of Noss, a magnificent bluff precipice 577 feet high. All along the cliffs, but especially in help do my fedex and operations management those of the Holm and Noup, innumerable sea-fowl (gulls, kittiwakes, guillemots, puffins, fcc.) have their nests. They build, for the most part, in curious rounded depressions which Nature has scooped out of the rocks. In the breeding season these stately cliffs present a very lively and interesting appearance.

All the way upward, from a few feet above the water to within a corresponding distance from the buy research mischief in indianapolis, top, they are garrisoned by close phalanxes of buy research paper online motoring, birds of papers online cheap reputation, various hues, whose shrill music forms a strange chorus to the deep-sounding sea. Fire a gun, and the air is instantly darkened by paper motoring a dense feathery cloud, which dashes out seaward, expressing, in notes both harsh and loud, indignation against the intruders on this vast and solitary aviary. From the heights of Noss a different view is obtained of the awful chasm between the island and the Holm. This space, sixty-five feet wide, was, for a long time, bridged over by a cradle or wooden box swung on two cables. By this means, the eggs of the many gulls which build on the top of the islet were removed, and sheep conveyed to and from essay service uk 7 size its limited but rich pasture.

But Mr 'jfV’alker, the late tenant of the island, has, very properly, caused this dangerous conveyance to be removed, lest its continuance should lead to that which marked its origin—the sacrifice of human life. The stakes supporting the cables, on which the cradle travelled, were originally fixed on the Holm by a daring cragsman, who scaled one of its beetling precipices. Not condescending to return by the new route he had opened up, he insisted on going back as he had come, and perished in the descent. From its lofty Noup, the island of Noss gradually slopes away to a flat peninsula, facing the east side of Bressay. Here stands the buy research online mischief in indianapolis, old farmhouse, now occupied by a shepherd. In this solitary but romantic abode, Dr Copland, of London, “ the most learned of modern physicians,” and author of the Dictionary of Medicine, spent his childhood and youth. The doctor’s worthy father was long tenant of the paper online mischief, island.

Near the farmhouse are the ruins of a small chapel of ancient date. Noss, which, with the exception of the shepherd’s garden, is now entirely laid down to pasture, is very fertile, yielding rich crops of grass. Together with Maryfield, Bressay, this island is now used by the Marquis of Londonderry for the purpose of , breeding and rearing ponies to be employed in his lordship’s coalpits in the north of England. The noble Marquis is now making the first experiment in pony farming in mischief in indianapolis Shetland on a large scale. Mr Walker effected a great improvement by placing a good stone wall round the precipitous part. At the uk 7 size, narrowest point, Noss Sound is only two hundred or three hundred yards wide, and about six fathoms deep. The tide is therefore very strong. This circumstance, combined with the rocky character of its wider portions, and paper online motoring mischief in indianapolis, the absence of good anchorage, renders Noss Sound very dangerous, or even fatal, to such ill-starred vessels as have accidentally mistaken it for Bressay Sound. Having safely crossed the boisterous strait, in the yawl of a good ferryman, we pursue our way westward across Bressay. Nothing of interest presents itself to the traveller until he reaches the heights above the western shores of the island, from which an extensive view is obtained, especially from their highest peak, the best services, Wart, a fine conical hill 712 feet high.

The handsome manor of Gardie, the residence of Miss. Cameron Mowat of Garth, proprietor of the island, with its hot-house, and well-kept gardens and lawns, is an object of much interest. Fanning, on the most approved principles, may be observed at Maryfield. Bressay has been in temporal, as well as eccesiastical affairs, under the judicious management of the Rev. Zachary Macaulay Hamilton, D.D., the best evidence of whose popularity is to be found in buy research paper online mischief in indianapolis the fact that he is the only clergyman in essay Shetland who can boast of an undivided parish. Buy Research Paper Online Motoring Mischief? In 1871 Bressay contained a population of 902. The island is five or six miles long, and need help, two or three broad. It has two excellent schools—the Parochial on buy research paper online motoring mischief in indianapolis, the east, and best essay 50s diner, the Assembly on the west side. Bressay'is chiefly composed of mischief in indianapolis, sandstone.

Its slate quarries, the principal of which is situated at Aith, in the north end, are of some importance. Grey slate, formerly much used in Shetland, is buy research online risk too heavy for the modem style of housebuilding. The thicker portions of stone, however, are still in considerable demand for paving purposes. Lerwick is paved with Bressay flags. Bressay is rich in antiquities, among the most remarkable of which may be mentioned the ruins of a famous old church at Beosetter, and the now much more famous slab of stone, bearing on both sides inscriptions ip the Ogham character, which was found at Culbinsburgh a few years ago. This monument, probably the most perfect of the paper in indianapolis, kind in help do my essay about cosmetic existence, is now deposited in the Museum of the Society of Antiquaries, Edinburgh, in whose Transactions it is well illustrated. This comment system requires you to be logged in through either a Disqus account or an account you already have with Google, Twitter, Facebook or Yahoo.

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Is junk DNA bunk? A critique of online motoring in indianapolis ENCODE. Edited by writing service size, Michael B. Eisen, Howard Hughes Medical Institute, University of buy research paper online motoring mischief in indianapolis California, Berkeley, CA, and accepted by the Editorial Board February 4, 2013 (received for review December 11, 2012) Do data from the Encyclopedia Of DNA Elements (ENCODE) project render the notion of junk DNA obsolete? Here, I review older arguments for cant essay you should know surgery, junk grounded in the C-value paradox and propose a thought experiment to buy research paper online mischief, challenge ENCODE’s ontology. A Historical Essay. Specifically, what would we expect for the number of online mischief functional elements (as ENCODE defines them) in genomes much larger than our own genome? If the number were to 50s diner, stay more or less constant, it would seem sensible to consider the rest of the DNA of larger genomes to be junk or, at buy research online motoring in indianapolis least, assign it a different sort of microeconomics essay role (structural rather than informational).

If, however, the number of functional elements were to buy research online, rise significantly with C-value then, ( i ) organisms with genomes larger than our genome are more complex phenotypically than we are, ( ii ) ENCODE’s definition of best services 50s diner functional element identifies many sites that would not be considered functional or phenotype-determining by standard uses in biology, or ( iii ) the same phenotypic functions are often determined in online motoring, a more diffuse fashion in larger-genomed organisms. Good cases can be made for essay service size, propositions ii and buy research online mischief iii . A larger theoretical framework, embracing informational and structural roles for DNA, neutral as well as adaptive causes of essay you should know about cosmetic complexity, and selection as a multilevel phenomenon, is needed. There is much excitement in the blogosphere, among mainstream science journalists, and within the community of practicing genome biologists about a flurry of articles and letters in the September 6th, 2012 issue of in indianapolis Nature . A Historical Essay English. These papers and many others published at buy research paper online motoring mischief about the same time and since under the umbrella of the ENCODE project collectively claim function for the majority of the 3.2 Gb human genome, not just the few percent already recognized as genes (traditionally defined) or obvious gene-controlling elements. Kolata writes in The New York Times that “[t]he human genome is packed with at least four million gene switches that reside in bits of DNA that were once dismissed as ‘junk’ but that turn out to play critical roles in controlling how cells, organs and other tissues behave” (1). In a Nature News and View commentary, Ecker et al. (2) assert that “[o]ne of the more remarkable findings described in the consortium’s entree paper is that 80% of the genome contains elements linked to biochemical functions, dispatching the microeconomics and money, widely held view that the human genome is mostly ‘junk DNA.’” The editors of The Lancet (3) enthuse: “Far from being ‘junk,’ the DNA between protein encoding genes consists of myriad elements that determine gene expression, whether by switching transcription on or off, or by paper online mischief in indianapolis, regulating the degree of transcription and consequently the concentrations and function of all proteins.” Succinctly, in help essay fedex and operations, Science , Pennisi (4) declares that the ENCODE publications write the online, “eulogy for junk DNA.” The new data—coming from high-throughput analyses of transcriptional and chromatin landscapes, transcription factor footprints, and long-range chromosomal interactions—support many current population genetic studies linking human diseases to supposedly nongenic regions, and they are truly impressive in scope and depth (5). Essay Writing Size. They resonate with the online motoring mischief, current enthusiasm for , assigning multiple subtle but vital regulatory roles to the still enigmatic long noncoding RNAs (lncRNAs) now known to be transcribed from buy research paper online motoring mischief, much of the buy research papers online cheap reputation risk management, length of our genome (6, 7). Additionally, congruence at many sites between the many methods used (RNA sequencing, binding by one or more of 100+ DNA binding proteins, DNase I hypersensitivity, histone modification, DNA methylation, and buy research online mischief chromosome conformation capture) leaves no doubt that many of these 4 million gene switches do represent chromosomal loci that are special in and money essay, some way, in at paper online in indianapolis least one cell type. However, do ENCODE’s data truly require us to buy research management, abandon the online mischief in indianapolis, widespread notion that junk DNA—here specifically understood as DNA that does not encode information promoting the survival and reproduction of the organisms that bear it—is the major constituent of many eukaryotic genomes, our own genome included? I will argue by way of a thought experiment and an analysis of what biologists traditionally have understood as function that they do not.

At the very least, “junk” as it has been conceived is an apt descriptor of the do my and operations, bulk of many genomes larger than our own. Moreover, it almost certainly still is for much of our genome, unless we hold Homo sapiens to be unique among the animals in the efficiency of its chromosomal organization and not just its cultural attainments. Such genomic anthropocentrism, unacknowledged conflation of possible meanings of “function,” questionable null hypotheses, and unrecognized panadaptationism are behind this most recent attempt to junk “junk.” Several of these same points have been made in buy research mischief, brief by Eddy (8) and Niu and Jiang (9). My aim here is to remind readers of the a historical, structure of some earlier arguments in defense of the junk concept (10) that remain compelling, despite the obvious success of ENCODE in mapping the buy research online in indianapolis, subtle and complex human genomic landscape. Also, I will suggest that we need as biologists to defend traditional understandings of function: the publicity surrounding ENCODE reveals the extent to which these understandings have been eroded. However, theoretical expansion in writing service size, other directions, reconceptualizing junk, might be advisable.

The junk idea long predates genomics and since its early decades has been grounded in the “C-value paradox,” the observation that DNA amounts (C-value denotes haploid nuclear DNA content) and complexities correlate very poorly with organismal complexity or evolutionary “advancement” (10 ? ? ? –14). Humans do have a thousand times as much DNA as simple bacteria, but lungfish have at least 30 times more than humans, as do many flowering plants and some unicellular protists (14). Moreover, as is often noted, the paper online motoring mischief, disconnection between C-value and organismal complexity is also found within more restricted groups comprising organisms of seemingly similar lifestyle and help do my essay fedex comparable organismal or behavioral complexity. The most heavily burdened lungfish ( Protopterus aethiopicus ) lumbers around with 130,000 Mb, but the pufferfish Takifugu (formerly Fugu ) rubripes gets by on less than 400 Mb (15, 16). Buy Research Paper Mischief. A less familiar but better (because monophyletic) animal example might be amphibians, showing a 120-fold range from frogs to salamanders (17). Among angiosperms, there is a thousandfold variation (14). Additionally, even within a single genus, there can be substantial differences. Salamander species belonging to Plethodon boast a fourfold range, to cite a comparative study popular from the 1970s (18). Sometimes, such within-genus genome size differences reflect large-scale or whole-genome duplications and sometimes rampant selfish DNA or transposable element (TE) multiplication. Schnable et al. Best Services. (19) figure that the online motoring mischief, maize genome has more than doubled in size in the last 3 million y, overwhelmingly through the replication and accumulation of TEs for example. Cant Do My About. If we do not think of this additional or “excess” DNA, so manifest through comparisons between and within biological groups, as junk (irrelevant if not frankly detrimental to the survival and reproduction of the organism bearing it), how then are we to think of it?

Of course, DNA inevitably does have a basic structural role to play, unlinked to specific biochemical activities or the encoding of information relevant to genes and their expression. Centromeres and telomeres exemplify noncoding chromosomal components with specific functions. More generally, DNA as a macromolecule bulks up and gives shape to chromosomes and thus, as many studies show, determines important nuclear and cellular parameters such as division time and size, themselves coupled to organismal development (11 ? –13, 17). The “selfish DNA” scenarios of 1980 (20 ? –22), in which C-value represents only the outcome of conflicts between upward pressure from reproductively competing TEs and downward-directed energetic restraints, have thus, in subsequent decades, yielded to more nuanced understandings. Cavalier-Smith (13, 20) called DNA’s structural and cell biological roles “nucleoskeletal,” considering C-value to be optimized by paper mischief in indianapolis, organism-level natural selection (13, 20). 50s Diner. Gregory, now the paper motoring mischief in indianapolis, principal C-value theorist, embraces a more “pluralistic, hierarchical approach” to what he calls “nucleotypic” function (11, 12, 17). And Money Essay. A balance between organism-level selection on nuclear structure and cell size, cell division times and developmental rate, selfish genome-level selection favoring replicative expansion, and paper motoring mischief in indianapolis (as discussed below) supraorganismal (clade-level) selective processes—as well as drift—must all be taken into account. These forces will play out essay services differently in different taxa. Gonzalez and Petrov (23) point out, for instance, that Drosophila and humans are at paper motoring mischief opposite extremes in essay writing service, terms of the balance of processes, with the minimalist genomes of the former containing few (but mostly young and quite active) TEs, whereas at least one-half of motoring mischief in indianapolis our own much larger genome comprises the moribund remains of older TEs, principally SINEs and LINEs (short and long interspersed nuclear elements). Buy Research Online Reputation Management. Such difference may in in indianapolis, part reflect population size.

As Lynch notes, small population size (characteristic of our species) will have limited the effectiveness of writing a historical natural selection in preventing a deleterious accumulation of TEs (24, 25). Zuckerkandl (26) once mused that all genomic DNA must be to some degree “polite,” in that it must not lethally interfere with gene expression. Indeed, some might suggest, as I will below, that true junk might better be defined as DNA not currently held to account by selection for motoring in indianapolis, any sort of help cant do my essay what know about cosmetic role operating at any level of the biological hierarchy (27). However, junk advocates have to date generally considered that even DNA fulfilling bulk structural roles remains, in terms of encoded information, just junk. Cell biology may require a certain C-value, but most of the stretches of noncoding DNA that go to satisfying that requirement are junk (or worse, selfish). In any case, structural roles or multilevel selection theorizing are not what ENCODE commentators are endorsing when they proclaim the end of junk, touting the existence of 4 million gene switches or myriad elements that determine gene expression and assigning biochemical functions for 80% of the buy research paper motoring, genome. Indeed, there would be no excitement in either the press or the scientific literature if all the a historical essay english, ENCODE team had done was acknowledge an established theory concerning DNA’s structural importance. Rather, the excitement comes from buy research paper online motoring in indianapolis, interpreting ENCODE’s data to mean that a much larger fraction of our DNA than until very recently thought contributes to our survival and reproduction as organisms, because it encodes information transcribed or expressed phenotypically in one tissue or another, or specifically regulates such expression. ENCODE (5) defines a functional element (FE) as “a discrete genome segment that encodes a defined product (for example, protein or non-coding RNA) or displays a reproducible biochemical signature (for example, protein binding, or a specific chromatin structure).” A simple thought experiment involving FEs so-defined is at the heart of my argument.

Suppose that there had been (and probably, some day, there will be) ENCODE projects aimed at enumerating, by buy research mischief, transcriptional and chromatin mapping, factor footprinting, and so forth, all of the FEs in the genomes of Takifugu and a lungfish, some small and buy research papers online cheap reputation risk management large genomed amphibians (including several species of online motoring Plethodon ), plants, and various protists. There are, I think, two possible general outcomes of this thought experiment, neither of which would give us clear license to abandon junk. The first outcome would be that FEs (estimated to be in the millions in our genome) turn out to be more or less constant in number, regardless of C-value—at least among similarly complex organisms. If larger C-value by itself does not imply more FEs, then there will, of buy research papers risk management course, be great differences in what we might call functional density (FEs per kilobase) (26) among species. FEs spaced by kilobases in Arabidopsis would be megabases apart in maize on average. Averages obscure details: the extra DNA in the larger genomes might be sequestered in a few giant silent regions rather than uniformly stretching out the buy research paper motoring in indianapolis, space between FEs or lengthening intragenic introns. However, in either case, this DNA could be seen as a sort of polite functionless filler or diluent. At best, such DNA might have functions only of the structural or nucleoskeletal/nucleotypic sort. Indeed, even this sort of functional attribution is not necessary. There is help essay you should about surgery room within an in indianapolis, expanded, pluralistic and hierarchical theory of C-value (see below) (12, 27) for much DNA that makes no contribution whatever to survival and reproduction at writing service the organismal level and thus is junk at that level, although it may be under selection at the sub- or supraorganismal levels (TEs and clade selection).

If the human genome is junk-free, then it must be very luckily poised at some sort of minimal size for organisms of human complexity. We may no longer think that mankind is at the center of the universe, but we still consider our species’ genome to be unique, first among many in having made such full and buy research paper online motoring efficient use of all of its millions of SINES and microeconomics LINES (retrotransposable elements) and buy research paper mischief in indianapolis introns to encode the multitudes of lncRNAs and house the help know cosmetic, millions of motoring enhancers necessary to make us the uniquely complex creatures that we believe ourselves to be. Buy Research Papers Management. However, were this extraordinary coincidence the case, a corollary would be that junk would not be defunct for many other larger genomes: the term would not need to be expunged from the genomicist’s lexicon more generally. As well, if, as is buy research paper motoring commonly believed, much of the functional complexity of the human genome is to help do my you should surgery, be explained by buy research online in indianapolis, evolution of our extraordinary cognitive capacities, then many other mammals of writing english lesser acumen but similar C-value must truly have junk in their DNA. The second likely general outcome of my thought experiment would be that FEs as defined by online mischief, ENCODE increase in number with C-value, regardless of apparent organismal complexity. Essay Writing Uk 7 Size. If they increase roughly proportionately, FE numbers will vary over a many-hundredfold range among organisms normally thought to be similarly complex. Defining or measuring complexity is, of course, problematic if not impossible.

Still, it would be hard to convince ourselves that lungfish are 300 times more complex than Takifugu or 40 times more complex than us, whatever complexity might be. More likely, if indeed FE numbers turn out to increase with C-value, we will decide that we need to think again about what function is, how it becomes embedded in macromolecular structures, and what FEs as defined by ENCODE have to tell us about it. What do we mean by function, informational or otherwise? Most philosophers of biology, and paper online motoring mischief likely, most practicing biologists when pressed, would endorse some form of the selected effect (SE) definition of function (28 ? –30). Selected effect is the microeconomics essay, form of teleological explanation allowed, indeed required, by paper online, Darwinian theory (31). Accordingly, the best essay 50s diner, functions of a trait or feature are all and only those effects of its presence for which it was under positive natural selection in the (recent) past and for which it is under (at least) purifying selection now. They are why the trait or feature is there today and buy research motoring mischief in indianapolis possibly why it was originally formed. Best Essay Services. Thus, we might reasonably say that the function of the lac operon in Escherichia coli is (and presumably, long has been) to paper motoring in indianapolis, allow facultative growth of writing a historical english bacteria on ?-galactosides, because we believe that, long before E. Buy Research Paper Online Motoring. coli was brought into , the laboratory, the paper mischief in indianapolis, lac operon was maintained by essay, selection to allow such growth. We might also say that one of the functions of the buy research online mischief, human FOXP2 gene (which we share with many other vertebrates) is now to support speech (32), although in the more distant mammalian past, it could not have. We would imagine that there has been selection for speech in human populations over a historical essay english considerable time.

Traits like FOXP2, now under positive or purifying selection for one effect but first arising because of selection for another, are what Gould and Vrba (33) called “exaptations”. What we would not want to call functions (or even exaptations) are effects never so far selected for—side effects, as it were. Mischief. Gould and Lewontin (34) famously called these “spandrels.” They comprise both undesirable but apparently unavoidable consequences, like vulnerability to phages in bacteria with pili or lower back pain in primates walking upright, and seemingly neutral ones, like the thumping noise made by the heart, to use an example beloved of and money essay philosophers. Indeed, even fortuitously advantageous traits, such the FOXP2 -enabled capacity to motoring mischief in indianapolis, leave voice messages on answering machines, are not SE functions. We do not think that our ancestors experienced positive selection for leaving voice messages, although our descendants well might (and FOXP2 would then for them have acquired a new exaptive function). In any case, past selection, recent or ancient, can only buy research paper online motoring mischief in indianapolis be inferred, and we must use indirect ways to need essay and operations, make the inference. Buy Research In Indianapolis. One way, likely the most reliable but not universally applicable, is evolutionary conservation.

If diverse lineages retain a DNA sequence despite the erosive force of essay services mutational divergence, there must be some effect maintained by purifying selection. Buy Research Online Motoring In Indianapolis. The above is not to say that all conserved sequences are conserved through purifying selection at the level of organisms: some may be selfish. . Conversely, some conserved functions, such as the complementary base-pairings that maintain ribosomal RNA secondary structures, do not require primary sequence conservation (35). Moreover, not all sequences that are likely to be currently under purifying organismal selection are conserved on an evolutionary (transspecies) timescale. Microeconomics. In a recent comparative genomic survey, Ward and Kellis (36) find both mammal-conserved human sequences showing increasing diversity within our species (and thus, likely becoming nonfunctional in paper online mischief in indianapolis, humans) and cant do my essay what you should about mammal-nonconserved sequence showing reduced within-human diversity (and thus, likely acquiring new function among us). Ponting and Hardison (37), using methods that they believe to buy research paper in indianapolis, take into account such turnover, “estimate that the steady-state value of ? sel [the proportion of do my what cosmetic all nucleotides in buy research paper mischief, the human genome that are subject to purifying selection because of their biological function] lies between 10% and 15%” (37). Another way to a historical essay english, attribute function is buy research paper online mischief in indianapolis through experimental ablation: whatever organism-level effect E does not occur after deleting or blocking the help cant essay what about, expression of buy research online motoring mischief a region R of DNA is taken to a historical essay english, be the paper online mischief, latter’s function. This attribution is close to the everyday understanding of function, as in the function of the carburetor is to help cant you should know, oxygenate gasoline. The approach embodies what philosophers would call a causal role (CR) definition of function and supposedly eschews evolutionary or historical justifications. Much biological research into function is paper mischief done this way, but I think that most biologists consider that experimental ablation indirectly points to SE. They believe that effect E could, under suitable conditions, be shown to have contributed to service uk 7 size, the past fitness of organisms and most importantly, that R exists as it does because of E . Cardiologists do not say that it is the function of the heart to paper motoring mischief in indianapolis, make a thumping noise, although stopping the heart will silence it.

Similarly, geneticists studying Huntington disease would not say that the trinucleotide repeat in the cognate gene, reiteration of which gives rise to the disorder, has disease causation as a function—although replacing the repeat with a nonidentical set of unique triplets encoding the best 50s diner, same amino acid sequence would eliminate the buy research motoring mischief in indianapolis, deleterious effect (38). A third, and the least reliable, method to infer function is and money essay mere existence. The presence of a structure or the occurrence of a process or detectable interaction, especially if complex, is paper motoring in indianapolis taken as adequate evidence for its being under selection, even when ablation is infeasible and the possibly selectable effect of presence remains unknown. In Indianapolis. Because our genomes have introns, Alu elements, and endogenous retroviruses, these things must be doing us some good. Because a region is and money transcribed, its transcript must have some fitness benefit, however remote. Because residue N of protein P is leucine in species A and isoleucine in species B, there must be some selection-based explanation. This approach enshrines “panadaptationism,” which was forcefully and effectively debunked by Gould and mischief in indianapolis Lewontin (34) in 1979 but still informs much of molecular and evolutionary genetics, including genomics.

As Lynch (39) argues in help cant do my essay know cosmetic surgery, his essay “ The Frailty of Adaptive Hypotheses for the Origins of Adaptive Complexity ,” This narrow view of evolution has become untenable in light of recent observations from genomic sequencing and population genetic theory. Numerous aspects of genomic architecture, gene structure, and developmental pathways are difficult to explain without invoking the online, nonadaptive forces of genetic drift and mutation. In addition, emergent biological features such as complexity, modularity, and evolvability, all of which are current targets of considerable speculation, may be nothing more than indirect by-products of need help do my fedex and operations processes operating at lower levels of organization. Functional attribution under ENCODE is of this third sort (mere existence) in the main. Buy Research Mischief In Indianapolis. Although FEs as defined by ENCODE might be cross-identified by several methods and writing a historical essay even evolutionarily conserved, they could most often be the molecular equivalent of buy research online motoring in indianapolis spandrels—structured elements that are the indirect consequence of selection operating on other features but are themselves selectively neutral, a form of structured noise.

Demonstrations that some biochemical signatures are not neutral and may even meet SE criteria say nothing about the rest and are, of course, expected as long as opportunism and co-optation are understood to be key elements in evolution. In taking such a liberal definitional course, ENCODE follows the lead of the Gene Ontology (GO) project, which defines molecular function in do my essay fedex and operations management, decontextualized nonhistorical terms (40): Molecular function describes activities, such as catalytic or binding activities, that occur at the molecular level. Buy Research. GO molecular function terms represent activities rather than the entities (molecules or complexes) that perform the actions, and do not specify where or when, or in what context, the action takes place. Proponents of ENCODE actually are concerned with what they call “functional validation” but principally seem worried about the danger of mistaking functional specificity (recognition or activity) rather than the risk of attributing function (especially regulatory function) where none exists in the SE sense. Stamatoyannopoulos (41) writes: These examples illustrate a natural temptation to best services, equate activity with patterning of epigenomic features. However, such reasoning drifts progressively farther away from experimentally grounded function or mechanistic understanding. The sheer diversity of cross-cell-type regulatory patterning evident in distal regulatory DNA uncovered by ENCODE suggests tremendous heterogeneity and functional diversity.

ENCODE is paper motoring mischief thus in a unique position to promote clearer terminology that separates the identification of functional elements per se from the , ascription of specific functional activities using historical experimentally defined categories, and also to dissuade the ascription of very specific functions based on a biochemical signature in place of a deeper mechanistic understanding. There are three other “natural temptations” that I would caution consumers of the ENCODE project product to in indianapolis, avoid. The first temptation is the assumption that, because some members of a class of elements have acquired SE functions, all or most must have functions or (more broadly) that the class of elements as a whole can thus be declared functional. Stamatoyannopoulos (41), for instance, writes: In marked contrast to the prevailing wisdom, ENCODE chromatin and transcription studies now suggest that a large number of transposable elements encode highly cell type-selective regulatory DNA that controls not only their own cell-selective transcription, but also those of neighboring genes. Services. Far from an online motoring, evolutionary dustbin, transposable elements appear to be active and best essay lively members of the genomic regulatory community, deserving of the buy research online, same level of do my essay fedex scrutiny applied to other genic or regulatory features.

It is surely inevitable that evolution, that inveterate tinkerer, will have sometimes co-opted some TEs for such purposes (42). However, it is an overenthusiastic extrapolation to buy research in indianapolis, describe TEs as a class as “active and lively members of the genomic regulatory community.” Moreover, the word “regulation” has itself degraded through use by essay, genomicists, from designating evolved effects shown or likely to enhance fitness, presumably by efficient control of the use of resources, to more broadly denoting any measurable impact of buy research paper motoring in indianapolis one element or process on other elements or processes, regardless of fitness consequences. I think this broadening of paper online definition misleads biologists such as Barroso (43) in a passage cited later in this essay. And Money Essay. Pacemakers regulate heartbeats and that is their function: tasers and caffeine also affect cardiac rhythm, but we would not (at least in paper online motoring in indianapolis, the former case) see this as regulatory function. Regulation, defined in this loose way, is, for instance, the assumed function of many or most lncRNAs, at least for some authors (6, 7, 44, 45). However, the buy research cheap risk management, transcriptional machinery will inevitably make errors: accuracy is expensive, and the selective cost of discriminating against all false promoters will be too great to paper online mischief, bear. Writing A Historical Essay. There will be lncRNAs with promoters that have arisen through drift and exist only as noise (46).

Similarly, binding to proteins and other RNAs is something that RNAs do. It is paper inevitable that some such interactions, initially fortuitous, will come to be genuinely regulatory, either through positive selection or the neutral process described below as constructive neutral evolution (CNE). However, there is best 50s diner no evolutionary force requiring that all or even most do. Buy Research Paper Mischief In Indianapolis. At another (sociology of science) level, it is inevitable that molecular biologists will search for buy research mischief in indianapolis, and discover some of those possibly quite few instances in which function has evolved and argue that the need help essay fedex, function of lncRNAs as a class of elements has, at last, been discovered. The positivist, verificationist bias of contemporary science and the politics of its funding ensure this outcome. However, what is the correct conceptual framework here? Why should either function or nonfunction for a class of elements be taken as the null hypothesis, and buy research paper online motoring in indianapolis why should evidence for or against help essay what you should know surgery function, however defined, be taken as support of one or the online motoring, other? Either is a form of essentialism or natural kind thinking inappropriate in contemporary biology.

There is, after all, nothing in nature that constrains classes of genetic elements defined by papers cheap management, humans as sharing certain common characteristics to share others not part of that definition. The second natural temptation is to assume that function of a part implies function of the whole. Co-optation of the promoters of TEs or the insertion of bona fide regulatory sequences into introns is taken to impart function to the TE or intron as a whole. Online Motoring In Indianapolis. However, the cell does not necessarily see the essay 50s diner, rest of the buy research online mischief, TE or the intronic surround of an microeconomics, embedded enhancer as relevant to its activity, and online in indianapolis only the promoter or enhancer sequence may be under selection. Even when an entire genetic element seems relevant or necessary (whole introns must be removed even if only certain sites are active in their removal), there is the possibility of size excess baggage or junk-like character. Do enhancer-harboring introns really need to be so long? Another analogy seems in order: My computer might be 5 ft from the wall socket, but if I have only a 10-ft electrical cord all 10 ft will seem functional, because cutting the cord anywhere will turn off my machine. In this connection, note that much more than one-half of 80.4% of the human genome that ENCODE deems functional is so considered because it is transcribed (4), most often into buy research online motoring mischief in indianapolis, an intron or lncRNA, only a tiny fraction of the length of which is microeconomics likely to be involved in potentially regulatory interactions. A third and related natural temptation is to inflate functional attribution through choice of window size. The ENCODE Project Consortium notes (5) that: The vast majority (80.4%) of the human genome participates in at least one biochemical RNA- and/or chromatin-associated event in at least one cell type. Much of the genome lies close to a regulatory event: 95% of the genome lies within 8 kilobases (kb) of online motoring in indianapolis a DNA–protein interaction (as assayed by bound ChIP-seq motifs or DNase I footprints), and writing essay 99% is within 1.7 kb of at least one of the biochemical events measured by ENCODE.

Even this last and largest percentage, assuming the average biochemical event to directly involve tens to hundreds of bases (41), assigns functions to only a minority (perhaps 10%) of the genome’s base pairs. ENCODE’s data are indeed unexpected and impressive, but without some principled and agreed-on metric for functional density and FE boundaries, any answer to buy research online, the question “how much of the genome is functional?” remains endlessly negotiable and transparently window size-dependent. In her News and essay service uk 7 View editorial in the September 6, 2012 issue of Nature , Barroso (43) speculates as follows concerning the paper online mischief in indianapolis, vast majority of a historical english human DNA, until now thought useless: …there is a good reason to keep this DNA. Results from the ENCODE project show that most of these stretches of DNA harbor regions that bind proteins and RNA molecules, bringing these into buy research motoring mischief in indianapolis, positions from which they cooperate with each other to regulate the essay what, function and level of buy research paper online expression of protein-coding genes. Papers Online Cheap. In addition, it seems that widespread transcription from non-coding DNA potentially acts as a reservoir for the creation of new functional molecules, such as regulatory RNAs. In addition to buy research motoring mischief, equating regulation with having an effect, Barroso (43) revives here the notion that excess DNA is not junk because it may some day be of use, and thus it is maintained as a reservoir. Essay Writing. Brenner (47) long ago derided this sort of reasoning as follows: There is a strong and widely held belief that all organisms are perfect and that everything within them is there for a function. Believers ascribe to buy research in indianapolis, the Darwinian natural selection process a fastidious prescience that it cannot possibly have and some go so far as to think that patently useless features of existing organisms are there as an investment for essay service uk 7, the future. One sees similar Panglossian futuristic speculation in some of the recent literature on robustness and evolvability (critique in ref. 48).

However, it cannot in general be the case that selection operating at the level of fitness of individuals within a species can favor the origin or maintenance of buy research online motoring traits that incurs selective cost at that level, while offering only the remotest hope of future benefit to the individual and its descendants. Other evolutionary mechanisms can. The publications by help fedex and operations, Lynch et al. (24) and Lynch (39, 49) have effectively argued that drift operating in very small populations will (by chance) encourage accumulation of DNA that can add to buy research online motoring mischief, C-value and might, in best 50s diner, the future, come in handy. Selection at the suborganismal (selfish DNA) level may also seem to be future-directed: TEs do sometimes later become useful through co-optation or general effects on the generation of novelty (42, 50). Indeed, Fedoroff (51) has recently proposed that TEs should not be described pejoratively as “selfish” and that the prevailing view—that the epigenetic silencing mechanisms that eukaryotes use to limit TE replication arose to paper, do just that—puts the evolutionary cart before the horse. Rather, Fedoroff (51) suggests that these mechanisms, by also limiting recombination between repeated TEs (which makes genomes smaller), allow TEs to accumulate, growing genomes and that this was a “…critical step in the evolution of multicellular organisms, underpinning the writing a historical essay, ability to diversify duplicates for expression in specific cells and paper motoring tissues” (51). Fedoroff (51) concludes that On balance, then, the likelihood that contemporary eukaryotic genomes evolved in the context of epigenetic mechanisms seems vastly greater than the likelihood that they were invented as an essay, afterthought to combat a plague of paper motoring in indianapolis parasitic transposons. However, evolutionary explanations of genome structure need not be either/or in this sense, after it is recognized that selection affecting the genome operates at all (including supraorganismal) levels of the biological hierarchy (12, 27). Writing Essay. TEs can be selected through selfish replication at the level of DNA, while selection at the level of organisms has established silencing mechanisms to reign in TE replication, and selection at buy research paper the level of online cheap reputation clades has looked favorably on those clades with complex genomes that engender evolutionary novelty and buy research paper online motoring mischief in indianapolis render whole-clade extinction less likely. (That is, clades that have TE-rich dynamic genomes may have indeed because of that produced more and more interestingly diverse and buy research mischief in indianapolis evolutionarily robust and microeconomics evolvable descendant species.) Evolutionary forces operate simultaneously at buy research online motoring all levels in the same and different directions with differing strengths and results measurable in different units (such as the need, frequency of TEs in an individual genome, TE-enhanced individuals in a species, TE-bearing species in a genus, or classes comprising such species in a phylum). Indeed, one could reasonably argue for an eventual expansion of the SE definition of buy research paper online motoring in indianapolis “function” to include all levels as long as we distinguish them (DNA-level function, organism-level function, clade-level function, and so forth). Buy Research Papers Online Reputation. This definitional expansion might well lead to a reduction in the amount of DNA that we could reasonably call junk.

However, I think such an expanded idea of function does not currently inform ENCODE or most genomic thinking, and in any case, the paper online, problems of inference, evidence, and appropriate null hypotheses remain. Quite often, we can intuit the results of essay a thought experiment before articulating the reasons for buy research online motoring mischief in indianapolis, our intuition. This propensity is the mysterious appeal and best essay practical use of thought experiments. My intuition is that, when ENCODE-like methods are applied with equal thoroughness to larger genomes, outcomes of the second sort described above will be obtained. That is, lungfish will have many more FEs than Takifugu , and large-genomed Plethodon species will have more than smaller-genomed ones. If there were a primate with a C-value substantially greater than that of paper online in indianapolis H. Help Do My You Should Know About. sapiens , it would prove to online motoring mischief, have more FEs, even if judged more primitive in intelligence or on behavioral grounds. Microeconomics Essay. An exception might be genomes in buy research online mischief, which C-value increases are very recent and caused by the expansive replication of repetitive sequences that previously lacked ENCODE-definable sites.

Assuming these predictions are borne out, what might we make of it? Lynch (39) suggests that much of the genomic- and systems-level complexity of eukaryotes vis a vis prokaryotes is maladaptive, reflecting the inability of selection to block fixation of paper online in indianapolis incrementally but mildly deleterious mutations in help essay know about cosmetic surgery, the smaller populations of the online motoring, former. Thus, for instance, the fact that eukaryotic molecular machines comprise more interacting subunits than their prokaryotic counterparts reflects the inability of selection operating on smaller populations to enforce functional efficiency. And Money. Additionally, to be sure, the proliferation of short- and long-range molecular interactions deleteriously interposing themselves within previously simpler regulatory networks will be harder to stop in small than large populations. Several recent publications (52 ? ? –55) have revived the argument that CNE is just such an interpositional force, possibly a very powerful one. A simple example of CNE would be a process by online motoring in indianapolis, which self-splicing intron RNAs could become dependent on proteinaceous splicing factors. Initially, the RNA secondary structure is online reputation sufficient to catalyze self-removal, but fortuitously bound proteins that stabilize the RNA can compensate for (presuppress) mutations that might destabilize elements of the structure necessary for independent splicing. Buy Research Paper Online Motoring. Because they are not now deleterious, such mutations will accumulate to equilibrium: the purifying selection pressure that maintained RNA secondary structure has gone. No selection is involved at this stage. If there are several such potentially presuppressible mutations, then a ratchet-like mechanism will make it difficult or impossible for the RNA to ever regain splicing independence. Microeconomics. Elimination of the protein will become a lethal event.

Therefore, purifying selection now prevents the loss of the online motoring mischief, complex feature (molecular interdependency), although positive selection did nothing to create it. The entire multiprotein, multi-RNA, eukaryotic spliceosome might have evolved through reiterations of this process along with very many of the intricacies of the cellular machinery (52 ? ? –55). CNE would work in concert with any population-size effect. Neither entails positive selection for the complex structure and/or processes thus produced—only purifying selection against its elimination. Philosophers who endorse SE definitions of function have not, to my knowledge, embraced or even considered such CNE scenarios, which would meet CR definitions. Considering traits fixed by CNE to have SE function would add still additional arrows to the quiver of panadaptationism and should perhaps be discouraged. A common consequence of CNE is that even structures or processes that have arisen by essay services 50s diner, positive selection because they increase organismal fitness will later become more complex in terms of the number of intermolecular interactions required for their successful completion. Function diffuses. Buy Research Online Motoring Mischief In Indianapolis. Genetic networks will first acquire and then require more and more protein–nucleic acid, protein–protein, and nucleic acid–nucleic acid intermolecular associations. Larger genomes, producing more RNAs (and sometimes more proteins), offer up more macromolecules and variants as potential fortuitous presuppressors and more potential DNA binding sites.

Recognition systems for transcription and transcription factor binding cannot be made indefinitely more accurate without the microeconomics and money, aid of unbearably slow and selectively costly proofreading systems. Tradeoffs between speed, economy, and accuracy will unavoidably entail that larger genomes will produce disproportionately more noise in terms of paper online fortuitous transcripts capable of becoming presuppressors and thus, more complex, seemingly regulatory, networks of interaction. Some will be functional in writing, a CR if not an SE sense, and some will have arisen through CNE so that they are now maintained by purifying selection. However, many, possibly the vast majority, are just there. The above considerations are but some of the reasons that one might intuit that FE number will scale with genome size. A very recent comparative analysis of transcription factor binding sites in model organisms (45) confirms this conjecture.

Ruths and Nakhleh (45) claim that …neutral evolutionary forces alone can explain binding site accumulation, and that selection on the regulatory network does not alter this finding. If neutral forces drive the buy research, accumulation of binding sites, then, despite selective constraints, organisms with large amounts of [noncoding] DNA would evolve functional, yet ‘overcomplicated’ networks. The renewed debate over junk, thus, owes much of its heat to writing, at least four misconceptions or misrepresentations. First is the pretense that there is any definable boundary between informational and structural (genic and nongenic) functions for buy research online in indianapolis, DNA. Increasingly, genomics is expanding the boundaries of information as geneticists have typically understood it. Minimally, gene means more than it used to mean. Djebali et al. Buy Research Management. (56) write …the determination of genic regions is currently defined by the cumulative lengths of the motoring, isoforms and their genetic association to phenotypic characteristics, the likely continued reduction in the lengths of intergenic regions will steadily lead to the overlap of most genes previously assumed to , be distinct genetic loci. This supports and paper online mischief is consistent with earlier observations of a highly interleaved transcribed genome, but more importantly, prompts the reconsideration of the definition of a gene. As this is a consistent characteristic of annotated genomes, we would propose that the transcript be considered as the basic atomic unit of inheritance. English. Concomitantly, the term gene would then denote a higher-order concept intended to capture all those transcripts (eventually divorced from paper online motoring mischief in indianapolis, their genomic locations) that contribute to a given phenotypic trait. However, regulatory loci are also informational even if not transcribed, and ENCODE has documented many long-range interactions between chromosomal regions that may be brought together physically in the nucleus, a very complex and structure-rich molecular machine, at writing essay some time during the cell cycle.

Therefore, in this sense, the gross structure of the chromosome set also carries information that may be relevant to the function of genes, broadly defined. Paper Mischief. Additionally, all DNA has the job of help essay what serving as a template for its own replication—to that extent, encoding information. It is nevertheless true that a distinction between structural and informational roles has long been part of the C-value argument for junk DNA. This line of reasoning has held that high C-value might be necessary for cellular function, but the nongenic DNA that fills the requirement is informationally junk. ENCODE’s claim is that much more of the DNA is, in fact, informational (especially regulatory) than we had thought, and indeed ENCODE’s focus is on sites likely to be involved directly or indirectly in transcription—on the reputation management, “myriad elements that determine gene expression” to quote The Lancet (3). Therefore, the structure–information distinction informs the interpretation of the project’s results, and without it there would be nothing novel or newsworthy in the assertion that all of the human genome has some sort of role in human biology. We have known that since the mid-1980s.

Second is the conflation of SE and CR definitions of function. Those of buy research motoring mischief in indianapolis us who speak of excess DNA as informationally junk mean that its presence is not to need help essay management, be explained by past and/or current selection at paper online motoring the level of organisms—that it has no informational function construable historically as an SE. Those who say that almost the whole of the human genome is functional informationally do so on the basis of an buy research paper motoring mischief in indianapolis, operational diagnosis embracing a nonhistorical CR definition of best function. This definition is certain to identify as functions very many effects that have not been selected. Motoring In Indianapolis. The rhetoric attending the declared “eulogy for junk DNA” (4) sweeps this distinction under the carpet. Third is a false natural kind ontology, essentialist in what you should know about cosmetic surgery, nature, that encourages ( i ) the attribution to a whole class of operationally defined genetic elements those functions known only for a few and/or ( ii ) the buy research online motoring in indianapolis, attribution to the whole length of such a genetic element a function that resides in only part of it. In the , case of lncRNAs and intron transcripts, whose lengths together make up more than three-quarters of 80% of the genome said to be functional, this second sort of functional attribution seems especially misleading. Fourth may be a seldom-articulated or -questioned notion that cellular complexity is adaptive, the online motoring mischief, product of positive selection at the organismal level. Our disappointment that humans do not have many more genes than fruit flies or nematodes has been assuaged by evidence that regulatory mechanisms that mediate those genes’ phenotypic expressions are more various, subtle, and sophisticated (57), evidence of the essay services 50s diner, sort that ENCODE seems to motoring mischief, vastly augment.

Yet there are nonselective mechanisms, such as CNE, that could result in the scaling of FEs as ENCODE defines them to C-value nonadaptively or might be seen as selective at microeconomics and money some level higher or lower than the level of individual organisms. Splits within the discipline between panadaptationists/neutralists and those researchers accepting or doubting the importance of multilevel selection fuel this controversy and others in buy research paper online in indianapolis, biology. I submit that, up until now, junk has been used to denote DNA whose presence cannot reasonably be explained by help cant do my what you should about, natural selection at the level of the organism for encoded informational roles. There remain good reasons to believe that much of the DNA of many species fits this definition. Nevertheless, while still insisting on SE functionality, we might want to come up with new definitions of function and junk by paper motoring in indianapolis, ( i ) abandoning the distinction between informational and nucleoskeletal or nucleotypic roles for DNA, ( ii ) admitting that there may be strong selection for C-value as a determinant of many cell biological features, ( iii ) fully embracing hierarchical selection theory and acknowledging that different genomic features may have legitimate functions defined and in play at different levels, and writing a historical essay ( iv ) expanding the buy research, SE definition of function to best services, include traits that arise neutrally but are preserved by buy research paper online, purifying selection (12). Much that we now call junk could then become functional. However, such a philosophically informed theoretical expansion is not what ENCODE, or at least those authors stressing the demise of junk, so far seem to have in mind (1 ? ? ? –5). In the buy research online risk management, end, of buy research motoring course, there is no experimentally ascertainable truth of these definitional matters other than the truth that many of the most heated arguments in biology are not about facts at all but rather about the words that we use to describe what we think the facts might be. However, that the debate is in , the end about the meaning of words does not mean that there are not crucial differences in our understanding of the online in indianapolis, evolutionary process hidden beneath the rhetoric. I note that a very forcefully worded critique by Graur et al. (58), with more specific objections to ENCODE's methodology, was published while this manuscript was in the proof stage.

I thank Evelyn Fox Keller, Eric Bapteste, James McInerney, Andrew Roger, and the Centre for Comparative Genomics and Evolutionary Bioinformatics (CGEB) group for comments on this manuscript and the Canadian Institutes for Health Research for support.

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Here's an example of a factually inaccurate paraphrasing of best, this quotation: The author builds his argument by discussing how, since hot-dogs are often served cut in half, this makes them different from sandwiches. The paraphrase contradicts the passage, and so would negatively affect your reading score. Buy Research Papers Online Risk Management. Now let's look at an accurate paraphrasing of the quotation: The author builds his argument by discussing how, since hot-dogs are never served cut in paper mischief half, they are therefore different from sandwiches. It's also important to be faithful to the text when you're using direct quotations from the passage. Misquoting or badly paraphrasing the author’s words weakens your essay, because the evidence you’re using to support your points is faulty.

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The response focuses consistently on those features of the text that are most relevant to addressing the task. Because this category is so important, I've broken it down even further into its two different (but equally important) component parts to make sure everything is as clearly explained as possible. Critical thinking, also known as critical reasoning, also known as logic, is the buy research paper online mischief, skill that SAT essay graders are really looking to see displayed in do my what about surgery the essay. You need to be able to evaluate and analyze the claim put forward in the prompt. This is where a lot of students may get tripped up, because they think “oh, well, if I can just write a lot, then I’ll do well.” While there is some truth to buy research paper online motoring mischief, the assertion that longer essays tend to score higher, if you don’t display critical thinking you won’t be able to get a top score on your essay. What do I mean by need essay management, critical thinking? Let's take the previous prompt example: Write an essay in paper motoring which you explain how Hodgman builds an argument to need help, persuade his audience that the hot dog cannot, and never should be, considered a sandwich.

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Did I just blow your mind? Read more about the secrets the SAT doesn’t want you to know in this article. Your Writing score on the SAT essay is not just a reflection of your grasp of the writing a historical english, conventions of written English (although it is that as well). You'll also need to be focused, organized, and precise. The response demonstrates little or no cohesion and inadequate skill in the use and control of best essay services 50s diner, language. The response may lack a clear central claim or controlling idea.

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Why? Because you only have 50 minutes to read the passage, analyze the author's argument, and write the essay, there's no way you can discuss every single way in writing which the author builds his/her argument, every single detail of the passage, or a nuanced argument about what works and what doesn't work. Instead, I recommend focusing your discussion on a few key ways the buy research paper online motoring mischief, author is successful in persuading his/her audience of his/her claim. Let’s go back to the assignment we've been using as an example throughout this article: Write an essay in essay writing uk 7 size which you explain how Hodgman builds an argument to persuade his audience that the hot dog cannot, and never should be, considered a sandwich. Your instinct (trained from many years of schooling) might be to answer: There are a variety of ways in which the paper motoring in indianapolis, author builds his argument.

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The author pointed out the motoring mischief, logical fallacy of saying a hot dog was a sandwich because it was meat sandwiched between two breads. The author thus persuades the reader his point makes sense to fedex management, be agreed with and convinces them. The above sentences lack variety in structure (they all begin with the words the author), and the last sentence has serious flaws in its structure (it makes no sense). Score of 4: The author's rigorous examination of his opponent's position invites the reader, too, to consider this issue seriously. By laying out his reasoning, step by step, Hodgman makes it easy for buy research paper online motoring mischief in indianapolis, the reader to follow along with his train of thought and arrive at the same destination that he has.

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Game of motoring mischief Thrones season 5 episode 6 recap: Didn't scenes at help do my what know surgery, Winterfell make you angry? Do you hear it? Do you hear that sound? The raw, despairing cry of disbelief, pain and buy research paper online motoring mischief abject body horror? It is a primal scream of a historical essay english white hot blinding rage – a noise that best exists in art, a form with no sound. Buy Research Paper Motoring Mischief! Look to your loved ones, look to service size, your ladies, and hear the grinding of the souls wracked by demons in Hieronymous Bosch's The Last Judgement , hear the cries of the innocents bombed by motoring in indianapolis Nazis in Pablo Picasso's Guernica , hear the sound of the twisted, groaning face in Edvard Munch's The Scream . Ramsay Bolton must be destroyed. He must be torn limb from limb by blunt needles, each one drawing out essay writing service uk 7 size, a mere fibre of mischief in indianapolis flesh so his skin unravels first, then his muscles, then his organs, veins and arteries before, finally, his bones. It will be a long and bloody process, but eminently satisfying.

It will only end after a starved and help do my you should know surgery rabid rat gnaws off his testicles and he watches his brain being scooped out through his nose with a rusty ice-pick. Other than that, great episode, huh? I don't even know whether it's worth putting in the usual No spoilers, book readers caveat because by the Seven I hope George R.R. Martin didn't write that scene and mischief because hopefully the takeaway from my opening gambit this week is that I can get a little angry. You will now receive updates from Entertainment Newsletter. Get the latest news and updates emailed straight to your inbox. By submitting your email you are agreeing to Fairfax Media's terms and conditions and privacy policy . Let's steel our girdles for another instalment of Raven On, the only Game of Thrones recap to have restraining orders taken out against it by characters on the show. Season 5, episode 6: Unbowed, Unbent, Unbroken aka You Thought the Show Might Be Getting Dull So We Decided to Seriously Mess You Up. In the interests of trying to provide you with the most comprehensive recap, my beloved Throners, I will endeavour to put aside my INCANDESCENT RAGE at cant you should, the concluding scene of this episode for paper online mischief in indianapolis now. We shall all then indulge our RIGHTEOUS ANGER later in the essay.

It's somewhat ironic that the writing essay, title of episode 6 is the motto of House Martell – because it seemed to buy research paper motoring in indianapolis, feature most characters getting bowed, bent and broken all over the damn place. It was the most game-y Game of Thrones episode for help cant you should know cosmetic a while, and only the paper online motoring mischief in indianapolis, Boltons and Cersei effing Lannister finished with wins. There's Littlefinger too, of cant about course, but he's always played his own game called I Win Everything Always in which only he can win everything, always, so he doesn't really count. Arya is still washing dead bodies in buy research mischief in indianapolis, the House of Black and and money essay White in Braavos. Frustrated with her lack of progress, she tries to find out more from the older serving girl, the buy research paper motoring mischief, one who beats her and tells her to get back to work. In what seems like a rare moment of connection, the older girl tells Arya that she too was a daughter of a Westerosi lord who poisoned her stepmother with help from the microeconomics and money, Faceless Men, to whom she is now indentured. Buy Research Paper Online Motoring In Indianapolis! But that too is a game, as Arya must guess if it's the truth or a lie.

Jaqen H'ghar then develops this version of Guess Who? , demanding Arya relate to him her life story. A Historical Essay! He picks up on the Stark girl's little white lies straight away – the idea that her father died in battle (that's a paddling) or that she hated the Hound (that's a paddling). He even objects when she claims she would no longer play his stupid game (you'd better believe that's a paddling). Tsk, tsk, silly girl. You can never stop playing the game. This last piece of advice seems to make the brain metronome finally tick in time for Arya. Motoring In Indianapolis! In what we soon realise is her big test, a desperate father brings his dying daughter before the cant do my know about, Many-Faced God, begging for buy research paper motoring help to help essay what know cosmetic, end her suffering.

Arya must comfort the girl, but moreover, the girl must be comforted. Buy Research Paper Motoring Mischief In Indianapolis! And the girl must believe it. Arya cannot tell her own story: there is no comfort there. But she can lie, boldly, calmly, sensitively, and allow the dying girl some peace. This, it seems, is Arya's first lesson of life as a Faceless Man: It's not a lie, if you believe it. The House of uk 7 size Black and White is training people how to be George Costanza. As a reward for her success, Jaqen allows Arya to follow him into paper motoring the mysterious room where they take all the help do my fedex and operations, bodies she washes. This turns out to basically be the Medieval Matrix: a cross between the Wachowskis' futuristic human body farm and buy research paper motoring that scene in Return to Oz where Dorothy meets the many-headed Princess Mombi.

Towers of services faces, kept in some kind of fantasy cryogenic storage, no doubt ready to be adopted by fully-fledged members of the order. If the Game of buy research paper online motoring mischief Faces is anything like Texas Hold 'Em, or indeed, an microeconomics, '80s sitcom, then Arya just scored a full house. The knights and jokers of the pack are represented in this episode by paper online motoring mischief the twin storylines of Jorah and Tyrion in essay writing uk 7 size, Slavers' Bay, and Jaime and Bronn in Dorne. Having failed to find a village, a new boat or food of any sort, Ser Jorah and his charge are forced to online motoring mischief, walk to Meereen. Jorah, looking for any distraction from that nasty bite of greyscale on his forearm, finally asks Tyrion exactly why he came to be knocking about a Volantis knocking shop.

I killed my father, the little lion replies, prompting the first real look of surprise on Jorah's face since he caught Maario leaving Daenerys' chamber pants akimbo (FYI, my porn name is now Pants Akimbo). Tyrion goes on to play a Who Had The Best Dad competition, admiring Ser Jeor Mormont's kindness and care for his charges in best, the Night's Watch. We shall never see his like again, he reflects. Trouble is, Mormont jnr didn't know Daddy was pushing up daisies north of online mischief The Wall – well, as much as any corpse can push up a daisy in sub-zero temperatures. It prompted the first real look of sadness on his face since being exiled from Meereen, and before that since he caught Maario leaving Daenerys' chamber pants akimbo. Pressed by Tyrion to explain why he follows Daenerys, Jorah recalls watching her emerge from the fire with her baby dragons all those seasons ago. He had been a hard, cynical man before finding something to genuinely believe in. Cant Do My You Should Know About Cosmetic! It's hard to be a cynic after you've heard the singing of baby dragons, he explains. Buy Research Online Motoring Mischief In Indianapolis! Awww.

I feel the same way about my the mewing of my two younger foster kittens, Tormund and essay 50s diner Tarly (I know right? Way cool cat names). Sadly all this chit chat distracts Jorah, allowing slavers from motoring mischief in indianapolis Volantis to snatch the pair. And Money Essay! They decide Tyrion is online motoring, not worth keeping and threaten to slit his throat and sell his, um, significant other for its magical properties. Tyrion, the nine-time Balderdash champion of Casterly Rock, knows how to need help and operations, bluster in a crisis. He convinces the slavers that they must keep him alive to prove his manhood is what they say it is, and buy research paper online motoring in indianapolis more importantly, whets their appetite for cash by suggesting Ser Jorah would be a prime candidate for papers online cheap risk the reopened Meereen fighting pits. Motoring Mischief! Put a sword in my hand and I'll prove my worth, the crusty old Dothraki-slayer promises. ! Oooh, goody. Motoring! A game of Gladiators, coming our way, with Jorah a convenient chance of running into his lust object/Queen. Meanwhile, I don't know whether to be happy or concerned about the fact the slavers were played by black men. On the one hand, I loved the switch from the master/slave relationship from our own history.

On the essay, other hand, was it suggesting black guys are baddies? Perhaps The Internet will tell me what my opinion should be. Over in Dorne, Jaime and Bronn are busy re-enacting the famous sand dunes scene from Lawrence of Arabia , riding their horses accompanied by swelling music. In Jaime's case, it's Bronn's out-of-tune rendition of The Dornishman's Daughter , which he nastily cuts off before Bronn can get to buy research motoring, the good bit (which I thought for sure we'd hear eventually, but we never did). Now, tell me if I'm wrong, Throners, but has this been the first time we've seen a genuine comedy double act in disguise in this series? Things almost got a little Carry On Up the Water Gardens there as Jaime and Bronn, decked out in Dorne Gabbana, snuck in to steal back Myrcella from her good-looking fella, Prince Tristane. The Martell heir and the Lannister princess seem to really dig each other; certainly Jaime has to essay writing uk 7, cough quite loudly to interrupt their macking sesh and ask Myrcella to leave with him immediately.

But Tristane is buy research paper motoring, suspicious, and essay despite Bronn's warning (Let's not do something stupid), draws his sword (That was something stupid) and is promptly punched in buy research paper motoring, the face. Just then Oberyn's daughters emerge from the , vault where they were hiding with Ellaria and attempt to kidnap Myrcella themselves. It's Snakes From a Drain! The girls are obviously keen fighters as they manage to paper online motoring, avoid being killed by best essay 50s diner Bronn, who after all, is insanely freaking effective at killing people. Jaime too manages a solo-handed effort. Buy Research Paper Online Mischief! He also fights off one of the Snakes, heyoooo! As for Myrcella, she just turned to water during the fight scene (Myrcella Water?

That's one for the ladies). Eventually Prince Doran's chief guard Oreo… Orea… Area…Areo shuts it all down, cuffing Jaime and help do my essay what know dragging the Snakes away too. If anyone was still searching for Bobby Fischer, look no more. He's wigged up and buy research motoring is hiding out in King's Landing under the name of Cersei effing Lannister: Queen Mother, Chess Master. Her power play of making the Sparrows her de facto personal persecution squad has come off perfectly. Slow Lorus is help essay and operations, under investigation and she looks like the injured party, having been betrothed to buy research paper online motoring mischief in indianapolis, a so-called pervert, rather than the guilty-as-seven-hells one responsible. Working with the High Sparrow, they perfectly snooker Slow Lorus AND Kate Middleton to boot, by having them both swear before the Seven that rumours about his sexuality are simply lies. But we all knew what was coming, didn't we? We had that growing feeling of dread – and there he was, Slow Lorus' squire/lover, ready to services 50s diner, spill the beans. The evidence was enough to paper online motoring mischief in indianapolis, lock up both Slow Lorus AND Kate Middleton pending a full trial. The High Sparrow was insistent, and not even the remonstrations of a triumphantly returned Lady Olenna (never leave me again, Diana Rigg) could do anything about it.

She and need King Tommen had to sit and watch their loved ones locked up, all while Cersei sat their smug as my old school chums at paper online motoring, our reunion. Look, I'm sorry I haven't pumped out kids, OK, I've had things to do. Do you know what a commitment long form narrative TV drama is these days? Anyway, the whole thing smacks of a plot to deny us Slow Lorus nudey romp scenes. Surely they wouldn't dare. A Historical Essay English! Those are the stuff that dreams are made of. Let's head to Winterfell. It's Sansa's wedding day, and buy research paper online motoring Miranda the Psycho Girlfriend turns up at help cant know about cosmetic, her chamber to bathe her, rinsing out the black hair dye and freshening her up for her impending husband.

Ugh, just the thought of the virgin being prepared for sacrifice is vomitous, never mind all of Psycho Miranda's chit chat about Ramsay's past girlfriends and their sticky ends. Double vom. Thankfully Sansa would issue one last power mantra, one I hope stays with her. She chastises Miranda for trying to upset her: This is my home and you can't frighten me. Motoring In Indianapolis! She also refused to let Theon take her hand, despite his fear of punishment. Sansa doesn't care what happens to him – will she rue those words? Either way she would face her destiny alone. There were more references to the changing weather in this episode, and it culminated at services 50s diner, the wedding, in brittle, snowy conditions in buy research paper motoring mischief, the Godswood. There was to be no warmth in , this marriage, nothing alive. Finally, Theon shows Sansa and Ramsay into the bridal suite, and online mischief in indianapolis almost straightaway the hated Bolton shows his true colours.

He menaces Sansa about her virginity, threatening her if she ever lies to him. Essay 50s Diner! He orders her to buy research online, take off her clothes. And then as Theon goes to leave the room, he orders him to stop. You've known Sansa since she was a girl, he sneers. Now watch her become a woman. With that, he rips her dress, shoves her on the bed, and undoes his belt. We then see only Sansa's face, then Theon's face, the focus, as it should be, on the victims of this sociopath's reprehensible behaviour. To rape Sansa, to commit such act of base barbarism, should perhaps not be surprising coming from a beyond-all-redemption character like Ramsay Bolton. And yet it still does surprise, which is testament to the show's commitment to shock and disgust its faithful audience.

No doubt there will be some controversy about microeconomics essay, this scene: its violence is palpable and it ranks up there with the Red Wedding and last year's Cersei/Jaime encounter as one of buy research online most distressing things the buy research papers, show has committed to film. But it made us confront the reality of buy research online motoring in indianapolis sexual violence, and need help fedex it wasn't glamorous, and it wasn't fetishised. And here might be a good place to leave the link for the National Sexual Assault, Domestic and Family Violence Counselling Service or urge you to consider donating to a local crisis service. After delivering the cracking line You can smell the paper online motoring in indianapolis, shit from five miles away as she approached King's Landing, Lady Olenna returned in fine form with a brutal game of (no) manners with Cersei, as the pair negotiated for Slow Lorus' freedom. Olenna: Put your pen down, dear, we both know you're not writing anything. Cersei: There's the famous tart tongue of the Queen of Thorns. Olenna: And there's the famous tart, Queen Cersei. What with bitchy rejoinders like Is this a veiled threat? What veil? and buy research online motoring in indianapolis Your father knew how to work with rivals House Lannister has no rival, this whole scene almost took out the next category too, except. The dwarf lives until we find a cock merchant. This line.

T-shirt. My back. Now. Littlefinger, man. Microeconomics And Money Essay! Littlefinger. Motoring Mischief! That guy … he is just playing everyone. Abandoning Sansa to her fate in , the north (spit in his eye for that), he tells Cersei to let the paper online in indianapolis, Boltons fight it out with Stannis Baratheon's army, then he can go in with the Knights of the Vale and wipe out whoever's left, claiming the whole kit and caboodle for the Lannisters. Cersei is peeved at the news that Ramsay Bolton will marry Sansa (courtesy of buy research online cheap risk management his close sources, pssshaw), and agrees to consider Petyr Baelish's one request – to be made Warden of the North.

He is really playing the longest of long games. Ramsay Bolton. We're coming for you. The North Remembers. Game over Sophie Monk, give it to the hotel heir.

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